Toxicity of anti-dog lymphocyte serum to normal and leukemic human lymphocytes.

By ROBERT SCHREK, FREDERICK W. PRESTON AND ALBERT A. DIErz A PRELIMINARY REPORT’ showed that rabbit anti-dog lymphocyte serum was more toxic in vitro to leukemic than to normal human lymphocytes. This paper presents detailed data on the toxicity of this immune heterobogous serum. As the dog lymphocytes have the Forssman antigen and rabbits are Forssman negative,2 it was necessary to consider whether the observed toxic reaction of the immune serum was due to Forssman antibody. To study this question, other immune heterologous sera were prepared and tested for their toxicity to leukemic and normal human lymphocytes.